|T7-MS1||50,000 units/ml||5,000 units|
|T7-MS2||50,000 units/ml||25,000 units|
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology.
1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
Isolated from E.coli carrying a plasmid which contains the T7 RNA Polymerase gene
Protocol for Standard RNA Synthesis
|10X Reaction Buffer||2 µl|
|NTP||X µl||0.5 mM each|
|Template DNA||X µl||0.2–1 µg|
|RNase Inhibitor (optional)||0.5 µl||1 U/µl final|
|Fresh DTT (optional)||X µl||5 mM final|
|T7 RNA Pol||2 µl|
|Total Volume||20 µl|
Incubate at 37°C for 1 hour. For shorter (< 300 nt) transcripts incubate at 37°C for 2–16 hours.